Initially, we used in silico data to assess hedgehog and hippo connected genetic players involved in the activation of HSCs (TEAD2, FOXA2, LATS2), there epigenetic modifiers (miR-650 and RPARP AS-1 LncRNA) and HSCs effecter proteins (IL-6 and TGF-β).We then compared the impact of Mutaflor® on expression of these factors in sera and tissue of NASH model rats and healthy controls. The gene discussed is IL6; the disease is metabolic dysfunction-associated steatohepatitis.