As a result, we are the first to find that 16 of the 20 amino acids were increased in the supernate of HCT116 cells after silencing of SLC6A14 with siRNA or blockade with α-MT, demonstrating an important role of SLC6A14 in the regulation of amino acid uptake in CRC cells, which are essential for cancer cell proliferation. This evidence concerns the gene SLC6A14 and colorectal carcinoma.