Successively, a targeted gene panel sequencing (480 genes for orofacial clefts) revealed a novel de novo heterozygous c.262A > G mutation in exon 4 of IRF6 gene (OMIM *607,199) causing an asparagine to aspartate missense mutation (p.Asn88Asp) that was compatible with the phenotype. Here, IRF6 is linked to orofacial cleft.