To verify its role in endothelial dysfunction, endothelial cells were transiently transfected with EZH2 siRNA (si-EZH2), or negative control (si-NC), the results showed that compared with si-NC, si-EZH2 increased the expression of eNOS and reversed the upregulation of VCAM-1 and vWF induced by IS stimulation (Fig. 6J). The gene discussed is NOS3; the disease is endothelial dysfunction.