To identify the components that induced the increase of ANRIL in CKD serum, we then incubated endothelial cells with IS, HA, IAA and Hcy, and found that uremic toxins could increase the expression of ANRIL dose-dependently, which suggested that the upregulation of ANRIL in CKD was due to or at least partly due to uremia toxin. This evidence concerns the gene CDKN2B-AS1 and uremia.