We knockdown the expression of ANRIL in endothelial cells under IS stimulation, and the results showed that compared with scramble, ANRIL shRNA lentivirus infection (Sh-ANRIL) lead to eNOS expression upregulated, while the expression of VCAM-1 and vWF decreased (Fig. 4G). This evidence concerns the gene CDKN2B-AS1 and lentivirus infection.