HSF1 and neoplasm: Tumor formation by MeWo cells expressing hHSF1-S326A or hHSF1-S419A was significantly less than that by cells expressing wild-type HSF1; importantly, tumor formation by hHSF1-S326A/S419A-expressing cells was markedly less than that by single mutant-expressing cells (Fig. 7f, g and Supplementary Fig. 6e).