In CRISPR screens in our engineered APCtrunc system, however, the dependence on components of the Wnt signaling pathway was comparable in APCtrunc and APCWT cells in both RKO and HCT116 backgrounds (Fig 3D–F), indicating that introducing an APC truncation in Wnt‐low colorectal cancer cell lines, and thereby hyperactivating Wnt signaling, did not lead to a new dependency on Wnt signaling in the resulting APCtrunc cell lines. The gene discussed is APC; the disease is colorectal cancer.