Similarly, an elegant study by Obaid and colleagues using an NIR-II EGFR-targeting nanoliposome formulation to study the specificity of NP delivery to EGFR-expressing glioma cell lines in vitro, in vivo, and ex vivo, demonstrated that delivery of nanoliposomes to the tumor site did not correlate with EGFR expression in the three different glioma cell lines that were tested, underrepresenting the image-derived molecular specificity by up to 94.2% (Obaid et al., 2021). This evidence concerns the gene EGFR and central nervous system cancer.