The qRT-PCR results revealed that the expression of LOC100507144, LINC02269, and EBLN3P was upregulated, whereas the expression of MEG3, SNHG29, RB1-DT, LINC01970, FAM13A-AS1, and APOA1-AS was downregulated in ESCC tissues compared to that in normal tissues. The gene discussed is RB1; the disease is esophageal squamous cell carcinoma.