In this study, we applied a multidimensional immunofluorescence (MIF) method to detect the infiltrative levels of immune cells (CD4+, CD8+ and PD-1+ immune cells) and the protein expression of MxA (encoded by an interferon-stimulating gene MX1) and gp96 (glycoprotein 96, a major component of HSPPC-96 vaccine) in pre-vaccination GBM tissues and examined their value in predicting the therapeutic outcomes of HSPPC-96 vaccination. Here, HSP90B1 is linked to glioblastoma.