KRAS and colorectal carcinoma: Moreover, CRC harbouring mutant KRAS variant (KRAS G13D) formed more TNTs than CRC cells with wild-type KRAS. GFP-tagged mutant KRAS transferred via TNTs from CRC LOVO cells (expressing mutant KRAS) to HCT-8 cells (wild-type KRAS) was confirmed by fluorescence microscopy and fluorescence recovery after bleaching (FRAP) experiments.