To further investigate the influence of the STING agonist on the TIME of ROC1 cold tumors, we harvested ROC1-tumor tissues from mice in the PBS control and c-di-GMP treatment groups at the indicated times (Fig. 6c; orange arrows) and found that STING expression was increased after the intratumoral delivery of c-di-GMP (Fig. 7a). Here, STING1 is linked to neoplasm.