Selective up-regulation of Wnt/β-catenin signaling downstream target gene expression, such as Tcf4, Ccnd1, Axin2, and Lgr5, only revealed in hARtg+ luminal tumor cell branches in comparison to both the earlier trend of hARtg+ cells (cell fate 1) in pseudotime and the cell branch of hARtg− cells in trajectory expression plots, demonstrating the activation of Wnt/β-catenin signaling induced by hAR aligning with PCa development and progression. Here, TCF4 is linked to posterior cortical atrophy.