Increased expression of Wnt/β-catenin downstream target genes, including Tcf4, c-Myc, Ccnd1, Axin2, and Lgr5, was also identified in RNA samples isolated from pathologically confirmed PCa samples in comparison to PIN and prostate tissues from age and sex-matched wild type (WT) mice using qRT-PCR (Fig. 4g). This evidence concerns the gene LGR5 and prostate intraepithelial neoplasia.