Using pseudotemporal kinetic analysis, we further demonstrated increased expression of Tcf4, Ccnd1, Axin2, and Lgr5 in the later trend of tumor cells (fate 2) in comparison to the middle trend of cells (fate 1) in hARtg+ cell trajectory plots (Fig. 7e), showing the activation of Wnt/β-catenin signaling correlating with the course of tumor development. The gene discussed is TCF4; the disease is neoplasm.