If RGS6 can act through a mechanism not involving SMAD4 to suppress TGF-β-induced NSCLC EMT, overexpression of RGS6 in SMAD4-Cas9 cells should result in a combined and greater inhibition of TGF-β-induced NSCLC EMT events. Here, TGFB1 is linked to non-small cell lung carcinoma.