Consistent with our in vitro study, loss of RGS6 promoted TGF-β-induced NSCLC metastasis in vivo, indicated by significantly higher numbers of MOMNs in lung tissues from mice injected with RGS6-Cas9-1 or RGS6-Cas9-2 NSCLC cells (Supplementary Fig. 4B, C), as well as by significantly more micrometastases in lung (Supplementary Fig. 4D) and liver (Supplementary Fig. 4E) tissues from these mice. Here, RGS6 is linked to non-small cell lung carcinoma.