Next, in another model of LC, we used the classical chemical based (diethylnitrosamine; DEN) induction of LC with our liver-specific HKDC1 knockout mouse (HKDC1-LKO) model by crossing HKDC1 floxed (HKDC1f/f) mice [30, 33] with Albumin-Cre mice. This evidence concerns the gene ALB and laryngotracheoesophageal cleft.