To investigate the common features of the signaling pathway regulation between NOTCH3 and SMARCA4 in colon cancer cells, the CRC HT29 and HT115 cells (according to the relative mRNA expression of NOTCH3 in different CRC cells, cell lines with higher NOTCH3 transcriptional expression were selected; Fig. S1A) were used to knockdown NOTCH3 or SMARCA4 by transfecting the specific NOTCH3‐siRNAs or SMARCA4‐siRNAs, respectively. Here, NOTCH3 is linked to malignant colon neoplasm.