IFNG and infection: To determine if there is a brain region-specific difference between infection routes, we investigated the parasite burden and IFNγ expression in the whole brain, cortex, and hippocampus of mice at d28 p.i. Assessing parasite burden, we observed a non-significant increase in the parasite burden of whole brain, no change in the cortex, and a significantly higher parasite burden in the hippocampus of i.p. vs. p.o. infected mice (Figures 6B–D).