Furthermore, the hub bio-targets of calycosin against HF and DM were screened, namely, MAPK1, ARRB1, ABL1, CDK1, MAP3K3, STUB1, PPP1CA, STAT3, PML, IQGAP1, HSPA9, CAV1, KPNB1, SQSTM1, RUVBL1, SFPQ, PPP2CA, PCBP1, ANXA2, and PAK1. The gene discussed is PCBP1; the disease is hydrops fetalis.