LDLR and atherosclerosis: In our study, we used Ldlr−/−.Leiden mice, a specific substrain of conventional Ldlr-/- mice with a mixed 94% C57BL/6J and 6% 129S1/SvImJ background, and one of the few mouse models that develops both NASH and atherosclerosis when fed a high-fat diet (with a macronutrient composition akin to human diets and not supplemented with supraphysiological amounts of cholesterol).