In this research, the apoptosis induced by CNN1 was confirmed by phosphatidylserine externalization in K-562 (p < 0.001) and FEPS (p < 0.001) cell lines, similar findings were reported on in vitro models of breast cancer [56], lung adenocarcinoma [57], and colon carcinoma [33]. The gene discussed is CNN1; the disease is colon carcinoma.