The results showed that TRAF3IP2 was highly expressed in NONO-TFE3 tRCC tissues and cells, and the silence of TRAF3IP2 inhibited the proliferation, migration, and invasion of UOK109 cells which were derived from cancer tissue of patient with NONO-TFE3 tRCC. This evidence concerns the gene TRAF3IP2 and renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.