To determine whether glyphosate exposure equivalent to the levels detected in the brain increases the levels of hallmark AD-pathology Aβ and reduces cell viability, we isolated and plated primary cortical neurons from APP/PS1 pups and incubated them with either 0 μg/mL (vehicle), 10 μg/mL, 20 μg/mL or 40 μg/mL of glyphosate (Fig. 4A). The gene discussed is PSEN1; the disease is Alzheimer disease.