Correspondingly, in vivo treatment of tumors with JHU-083 had an increased the number of CD8+tumor-infiltrating lymphocytes (TILs), and their transcriptional program showed an enhanced proliferative capacity and anti-cancer activity, and the expression of genes related to long-term memory CD8+T cells was up-regulated, while the expression of genes related to apoptosis was significantly down-regulated. The gene discussed is CD8A; the disease is cancer.