Given that, we hypothesized that NK CD56bright, Tfh, and macrophage cell types might be closely related to immune regulation in TME of glioma, and B7H3, PDCD1, LAG3 and CXCL16, CXCR4, CCR5 might be key targets for glioma immunotherapy. This evidence concerns the gene CXCR4 and central nervous system cancer.