Given that, we hypothesized that NK CD56bright, Tfh, and macrophage cell types might be closely related to immune regulation in TME of glioma, and B7H3, PDCD1, LAG3 and CXCL16, CXCR4, CCR5 might be key targets for glioma immunotherapy. The gene discussed is PDCD1; the disease is central nervous system cancer.