FOXO1 and obesity due to melanocortin 4 receptor deficiency: Having documented that perinatal HFD with maternal obesity causes elevated serum IL-6 concentration and STAT3 activation in lungs, along with nuclear FoxO1 exclusion as well as SMC hyperproliferation, we next isolated primary bSMC from lungs of 21-day-old mice and exposed the cells to IL-6+sIL-6R (IL-6 + R) to investigate IL-6-STAT3-FoxO1 signaling.