To determine the impact of microbiota-derived EV on host sIgA production and translocation, we quantified the gene expression of CCL28, APRIL and PIGR in HT-29 cells incubated with vehicle control (PBS) or with small intestine microbiota-derived EV from mice fed on HP, HF and HC at physiological levels (HP > HC = HF). This evidence concerns the gene TNFSF13 and hydrops fetalis.