EDN1 and hepatocellular carcinoma: We detected the protein and mRNA expression of EDN1 in ARHGEF2 knockdown or overexpression HCC cells, showed that knockdown ARHGEF2 led to downregulation of EDN1 (Fig. 7F, Supplementary Fig. 4A), while overexpression with ARHGEF2 upregulated the protein and mRNA expression of EDN1 (Fig. 7G, Supplementary Fig. 4B).