We were able to create a stable HepG2 cell line with ARHGEF2 knockdown and an MHCC97H cell line with ARHGEF2 overexpression and found that ARHGEF2 could not only regulate the proliferation of HCC cells but also effectively stimulate the migration, invasion, and angiogenesis of HUVECs. The gene discussed is ARHGEF2; the disease is hepatocellular carcinoma.