ACE2 and infection: Regardless of the readout, the controls performed as expected, wherein VSV-G-coated pseudoviruses did not exhibit ACE2-dependent infection and miRFP670+ cells expressing a null ACE2 cDNA harboring an early frameshift mutation did not enhance spike-mediated pseudovirus infection above that of mCherry+ ACE2(dEcto) cells (Fig 5A).