To explore whether the protein level of MARCKSL1 is related to prognosis in lymph node‐negative ESCC patients, Kaplan–Meier survival analysis was also performed in ESCC patients with lymph node‐negative ESCC (n = 378) and showed that higher MARCKSL1 expression was markedly correlated with a poor survival rate (Figure 5H). This evidence concerns the gene MARCKSL1 and esophageal squamous cell carcinoma.