It has been shown that MARCKSL1 has a significant prognostic value in lymph node‐negative breast cancer,14, 18 hence, Kaplan–Meier survival analysis was performed in ESCC patients with lymph node‐negative disease, which showed that higher MARCKSL1 expression was markedly correlated with a poor survival rate (Figure 5H). This evidence concerns the gene MARCKSL1 and esophageal squamous cell carcinoma.