Therefore, to study if Rh4 inhibits the metastasis of ESCC by regulating EMT, we detected the expression of KYSE30, KYSE150, and KYSE410 related proteins including E-cadherin, N-cadherin, vimentin, and snail (with or without Rh4) (Figure 2C). This evidence concerns the gene VIM and esophageal squamous cell carcinoma.