CBD inhibits the activation of the EGF/EGFR signaling pathway and its downstream targets such as AKT, ERK and NF-kb, to inhibit the 4T1-mediated breast cancer cell line [22,27].CBD can also regulate the tumor microenvironment [28] and inhibit the migration, invasion and metastasis of aggressive breast cancer in vitro and in vivo [21,22,26,29]. Here, EGF is linked to breast cancer.