HSF1 and hepatocellular carcinoma: Then we used Western blotting of HCC cells incubated with 250 or 500 nM of CL-43 for 20 h with an antibody against HSF1 phosphorylated at S326 (phosphorylation of HSF1 residues S326 or S230 was found to convert HSF1 into a transcriptionally active trimer) [21,22,23] and with an antibody against the HSF1 direct target, Hsp70.