The 5XFAD mouse is a well-established model of EOFAD, carrying human APP and PSEN1 transgenes with a total of five AD-linked mutations: the Swedish (K670N/M671L), Florida (I716V) and London (V717I) mutations in APP, and the M146L and L286V mutations in PSEN1 [45]. Here, APP is linked to Alzheimer disease.