In our study, genistein treatment showed to greatly reduce the expression of Bcl-2 in all three cell lines whereas cFLIP tended to be decreased in KKU055 and KKU100 cells in a dose-dependent fashion but not in KKU213A cells (Figure 4c), which might lead to the apoptosis of these two CCA cells. This evidence concerns the gene BCL2 and cholangiocarcinoma.