Staining of tumor-draining LN sections for the fLEC/mLEC marker LYVE-1 and the sinusoidal macrophage marker CD169 revealed that the density of CD169+ macrophages in medullary sinuses was reduced in Pdpnfl/fl x Prox1-CreERT2 mice compared with Cre− control littermates, whereas the coverage of the subcapsular sinus by CD169+ macrophages was comparable in both groups (Figure 5f,g). This evidence concerns the gene PROX1 and neoplasm.