MSH6 and glioblastoma: To evaluate the cellular phenotype of MSH6 loss and the resulting TMZ resistance in GBM cells, we reduced the expression of MSH6 (knockdown, KD) using shRNA (Figure 1A) or mutated the MSH6 gene (knockout, KO) using the CRISPR/Cas9 system (Figure 1C) in LN428 glioblastoma cells.