The results showed that the oncolytic adenovirus Ad5F11bSP-Rantes could effectively mediate the expression of Rantes in breast cancer cells, and the levels of Rantes in supernatants of MCF-7 and MDA-MB-231 cells could reach 1683.26 pg/mL and 1628.99 pg/mL after 72 h of virus infection. This evidence concerns the gene CCL5 and breast cancer.