Based on these observations and the results from the previous gain and loss of functional studies in the field, showing the role of AKT1 in the cell survival and growth regulation [12,15]—the focus of the present study—we decided to examine the impact of selective depletion of AKT1 and the inhibiting of the AKT’s activity by inhibitor VIII on the genome-wide transcriptome of breast cancer cells using breast cancer MCF-7 cells as a model system, as these cells express abundant levels of AKT1 and are widely used for a large number of genome-wide discovery studies. This evidence concerns the gene AKT1 and breast cancer.