EGF and breast cancer: In general, we noticed a similar trend of increased or reduced expression of 11 tested transcripts, out of 21 selected genes, belonging to the pathways of interest to the laboratory, between the RNA-sequencing and qRT-PCR results in cells with siAKT1 (Figure 5A, Supplementary Figure S3) and among them 7 (without EGF) and 6 (EGF stimulated samples) genes were also found to exhibit the same pattern of expression in another breast cancer SKBR3 cell line (Supplementary Figure S3).