BSG and neoplasm: Using the RMS cell lines JR1 and Rh41, derived from an ERMS and an ARMS tumor, respectively [35,36], and using four different shRNA targeting CD147 (shCD147), and scrambled construct (shScr) as negative control, we found that the two constructs shRNA-11 (sh11) and shRNA-12 (sh12) exhibited the highest knockdown of CD147 by both protein expression (Figure 1A,B, with quantitation in Figure 1C,D), and RTq-PCR (Figure 1E,F), and therefore these two shRNA constructs were subsequently used throughout the remainder of this study.