As shown in Figure 6A–F, CTSD expression was highest in myeloid cells, high expression of AP1S1 was shown in glioblastoma cells, and YWHAG was mainly expressed in glioblastoma cells, oligodendrocytes, and myeloid cells, and IER3 was predominantly expressed in myeloid cells. The gene discussed is CTSD; the disease is glioblastoma.