The biochip was used to detect eight different cancer biomarkers: AFP, activated leukocyte cell adhesion molecule (ALCAM; breast cancer biomarker), cancer antigen 15-3 (CA15-3; breast cancer biomarker), cancer antigen 19-9 (CA19-9; pancreatic, colorectal, and ovarian cancer biomarker), cancer antigen-125 (CA-125; ovarian cancer biomarker), CEA, osteopontin (ovarian and liver cancer biomarker), and PSA. This evidence concerns the gene SPP1 and breast carcinoma.