Therefore, an in vivo analysis was carried out in normal rabbit to determine the relative pharmacological activity of insulin delivered through PIC-DNVs, INS-DNVs, and IPC-NVs, indicating that IPC-DNVs had sustained hypoglycemia as compared to INS-DNVs and IPC-NVs, with a high relative bioavailability (15.53%) as compared to that of NS-DNVs (3.09%) and IPC-NVs (1.96%). Here, INS is linked to Hypoglycemia.