Briefly, plasmids including transposase (pCMV(CAT)T7-SB100), human Akt1, and luciferase (pKT2/CLP-AKT-2A-OVA-HBc-HBs-LUC) and the N-Ras V12 mutant (pT/Caggs-NRASV12) were hydrodynamically injected (HDI) and the liver was monitored for the appearance of HCC. The gene discussed is AKT1; the disease is hepatocellular carcinoma.