To test this, CRC lines HCT-116 and DLD-1 were pretreated with resistin (50 ng/mL) or a vehicle (PBS) for 12 h, and then they were exposed to increasing doses of 5-FU (2.5–10 μM) in the presence or absence of resistin for an additional 48 h. This evidence concerns the gene RETN and colorectal carcinoma.