First, we analyzed lysates of drug-treated 2D and 3D cultures and tumor xenografts with LC3b and p62 (SQSTM1) antibodies, both of which have been identified as good indicators for autophagosome formation, and the content of LC3b-II is closely correlated with the number of autophagosomes [25]. The gene discussed is MAP1LC3B; the disease is neoplasm.