In order to verify whether glycine nano-selenium can improve the immune effect of H9 subtype avian influenza vaccine, the serum of mice in each group was collected at random at the end of the experiment, and the serum immunoglobulin IgG, IgM, IgA, and specific immunoglobulin aiv-h9 IgG were measured. This evidence concerns the gene CD40LG and avian influenza.