Alteration of proliferation, cell colony formation, migration, as well as the sensitivity towards different anticancer agents in stable CYP27C1-knocdown human lung cancer cells were further examined, which not only laid the foundations for revealing the mechanism of CYP27C1-associated lung cancer development, but also provided hints for identifying potential substrates of CYP27C1. This evidence concerns the gene CYP27C1 and lung cancer.