The results showed that compared with Sham rats, BDL rats showed significantly increased liver/body weight ratio, spleen/body weight ratio, as well as increased serum ALT, AST, ALP, total bile acids, total bilirubin, ammonia, and asymmetric dimethylarginine (ADMA), indicating that liver injury was successfully developed by BDL, and the BDL rats characterized hyperbilirubinemia (HB). This evidence concerns the gene GPT and hemoglobin measurement.