To assess the effect of JIB-04 on the stemness of liver CSCs, we investigated the mRNA expression levels of liver CSC marker genes, including CD44, CD133, CD90, LGR5, EpCAM, CD24, and CD13, in three HCC cell lines, namely PLC/PRF/5, Huh7, and HepG2, after JIB-04 treatment. This evidence concerns the gene PROM1 and hepatocellular carcinoma.