In particular, our data highlighted the importance of KDM6B in HCC malignancy and functions of LCSCs because the tumor-initiating ability of LCSCs was reduced by KDM6B deficiency (Figure 4F), and the JIB-04-induced elevation of H3K27me3 levels could be reduced by the demethylase activity of KDM6B [15]. Here, KDM6B is linked to hepatocellular carcinoma.