Based on our findings, we next questioned whether the PI3K-Akt signaling pathway was responsible for JIB-04-induced G1/S cell cycle arrest in HCC cell lines, as shown in Figure 1B. To achieve this, AKT protein levels in HCC cell lines after treatment with DMSO or JIB-04 were first analyzed with a Western blotting analysis using a pan-total AKT antibody. Here, AKT1 is linked to hepatocellular carcinoma.