To investigate the role of SPL in muscular dystrophy further, and with special attention to its early stages, we compared Sgpl1 gene expression by performing qRT-PCR on the skeletal muscles (gastrocnemius) of mdx and wild type (WT) C57BL/10 mice at 4, 6, 8, and 10 weeks of age (Figure 2A), which represents the peak period of myositis in the mdx model. Here, SGPL1 is linked to muscular dystrophy.