The flow cytometry analysis of PBMC-iNKT cells revealed enhanced cytotoxic function; compared to just PBMC-iNKT cells, PBMC-iNKT cells cocultured with tumor cells displayed greater levels of CD69, perforin, and granzyme B, while PBMC-iNKT cells cocultured with tumor cells and treated with αGC displayed greater levels of CD69, perforin, and granzyme B compared to both PBMC-iNKT cells cocultured with or without tumor cells (Figure 2E,F). The gene discussed is PRF1; the disease is neoplasm.