The human melanoma A375-CD1d-FG cell line and the human lung cancer H292-CD1d-FG cell line were generated by engineering their parental cell lines to overexpress human CD1d and the firefly luciferase (Fluc) and enhanced green fluorescence protein (EGFP) dual-reporters (FG) to allow for the convenient monitoring of the tumor cells through luciferase assays or flow cytometry (Figure 2B). This evidence concerns the gene CD1D and melanoma.